How to apply Selafort for rats?

How to apply Selafort for rats? - briefly

Administer Selafort orally at 10 mg/kg once daily, mixing the calculated dose into a small amount of palatable vehicle (e.g., sweetened water or gel) and confirming full ingestion before the next dose.

How to apply Selafort for rats? - in detail

Administering Selafort to laboratory rats requires precise preparation, dosage calculation, and monitoring to ensure reproducible results and animal welfare. The following protocol outlines each step in detail.

The compound should be dissolved in a suitable vehicle, such as sterile saline or a 0.5 % methylcellulose suspension, depending on solubility. Prepare a stock solution at a concentration that allows accurate pipetting of the required volume for the target dose. Filter the solution through a 0.22 µm membrane to remove particulates and store aliquots at 4 °C for no longer than 24 hours to prevent degradation.

Dose determination relies on the animal’s body weight. Calculate the amount of Selafort (mg) using the formula:

 Dose (mg) = Target dose (mg kg⁻¹) × Weight (kg)

Typical experimental ranges fall between 2 mg kg⁻¹ and 10 mg kg⁻¹; adjust according to the study’s objectives and prior literature. Record each rat’s weight immediately before dosing to minimize dosing errors.

Administration routes commonly employed are oral gavage and intraperitoneal injection. Select the route that aligns with the pharmacokinetic profile required for the investigation.

• Oral gavage:

  1. Load the calculated volume into a calibrated gavage needle (1 mL kg⁻¹ max).
  2. Restrain the rat gently, insert the needle along the esophagus, and deliver the solution in a steady stream.
  3. Observe the animal for regurgitation; repeat the procedure only if necessary.

• Intraperitoneal injection:

  1. Draw the dose into a sterile syringe with a 25‑30 G needle.
  2. Tilt the rat at a 30‑45° angle, locate the lower right quadrant of the abdomen, and insert the needle bevel up.
  3. Inject the solution slowly, withdraw the needle, and apply gentle pressure to the injection site.

After delivery, monitor the rats for at least 30 minutes for acute reactions such as respiratory distress, abnormal locomotion, or signs of discomfort. Record body temperature, heart rate, and activity levels at predetermined intervals (e.g., 0, 15, 30, 60 minutes). If adverse effects exceed predefined thresholds, halt the experiment and consult veterinary staff.

Maintain a detailed log that includes animal identification, weight, calculated dose, administration route, time of dosing, and observed post‑dose behavior. This documentation supports reproducibility and compliance with institutional animal care guidelines.

Finally, dispose of all waste according to biohazard protocols. Decontaminate reusable equipment with appropriate disinfectants before storage.