How to restrain a rat for an injection? - briefly
Hold the rat gently by the nape of the neck with a gloved hand, supporting its body and keeping the forelimbs folded against the torso to limit movement. Quickly locate the chosen injection site (e.g., dorsal flank or tail vein) and deliver the dose with a steady, controlled motion.
How to restrain a rat for an injection? - in detail
Properly securing a laboratory rat before delivering an injection reduces stress for the animal and improves the accuracy of the dose. Follow these steps to achieve safe and effective restraint.
Begin by preparing the workspace. Clean the surface, arrange all required supplies—syringe, needle, antiseptic swabs, and a soft restraining device—within arm’s reach. Verify that the injection material is at the correct temperature and concentration.
Select a restraint method appropriate for the procedure’s duration. Common options include:
- Tube restraint: Use a clear plastic tube slightly larger than the rat’s body. Gently guide the animal head‑first into the tube, leaving the tail exposed for handling. The tube limits movement while allowing visual monitoring.
- Scruff technique: Grasp the skin at the back of the neck with thumb and forefinger, lifting the animal slightly off the bench. This lifts the forelimbs and limits locomotion. Maintain the grip for only a few seconds to avoid excessive pressure.
- Manual hold: Position the rat on a padded surface, wrap one hand around the torso while the other secures the hindquarters. Keep the fingers close to the body to prevent escape.
While restraining, support the rat’s spine and avoid compressing the thorax. Maintain a calm environment; sudden noises can trigger defensive behavior. Once the animal is stable, disinfect the injection site with an appropriate antiseptic, allowing it to dry briefly.
Insert the needle using the recommended angle for the target tissue—typically 45° for subcutaneous injections and 90° for intramuscular delivery. Deliver the medication smoothly, then withdraw the needle at the same angle. Apply gentle pressure with a sterile swab to minimize bleeding.
Release the rat immediately after the procedure. Place it in a clean recovery cage with soft bedding, monitor for normal activity, and return it to its home cage once it regains composure. Document the injection details, including dose, site, and any observed reactions.