How should you properly handle mice? - briefly
Use clean, powder‑free gloves to gently grasp the mouse at the base of its tail while supporting its hindquarters, keeping handling brief and the environment calm to minimize stress. Sanitize equipment between animals and return the mouse to its cage promptly.
How should you properly handle mice? - in detail
When working with laboratory or pet mice, follow a systematic protocol to minimize stress for the animal and reduce risk of injury or contamination for the handler.
Prepare the environment. Clean the work surface with an appropriate disinfectant, then wipe it dry. Wear disposable gloves, a lab coat, and, if required, a face mask. Ensure that all tools—tweezers, forceps, or a handling tube—are sterilized and within easy reach.
Capture the animal. Use a clean cage or a small, transparent container to coax the mouse into a confined space. For quick removal, gently scoop the mouse from the cage floor with a pair of blunt-tipped forceps, avoiding pressure on the spine.
Restrain safely. Two common methods are:
- Tube restraint – place the mouse in a clear plastic tube, leaving only the head exposed. Hold the tube firmly but without squeezing, allowing the animal to breathe freely.
- Scruff technique – grasp the loose skin at the neck with thumb and forefinger, lifting the mouse slightly off the surface. Maintain a gentle grip; excessive force can cause injury.
Perform any required procedure—blood collection, injection, or measurement—while the animal remains calm. Use the smallest gauge needle suitable for the task, and keep the duration of restraint under one minute whenever possible.
Release the mouse. Return it to its cage promptly, providing fresh bedding and water. Observe for signs of distress such as rapid breathing, vocalization, or abnormal posture; if any appear, consult a veterinarian.
Maintain records. Document the date, procedure, personnel involved, and any observations of the animal’s condition. Store data in a secure, searchable system for future reference and regulatory compliance.
Sanitize after use. Discard gloves and other disposables in biohazard waste. Clean reusable equipment with an approved disinfectant and allow it to dry before the next session.
Adhering to these steps ensures humane treatment of mice, reliable experimental results, and a safe working environment.