How should you bathe mice properly? - briefly
Use a shallow container of lukewarm water (30‑32 °C) with a mild, non‑soap detergent, gently submerge each mouse for 30‑60 seconds, then rinse with clean water and dry with a low‑heat heat lamp. Ensure the mouse is handled minimally and kept warm throughout the procedure.
How should you bathe mice properly? - in detail
Proper bathing of laboratory mice requires preparation, gentle technique, and post‑procedure care to minimize stress and prevent injury.
Begin by assembling all materials: a clean, shallow container; warm water (30–32 °C); a mild, non‑ionic surfactant formulated for small mammals; sterile gauze or soft brush; absorbent towels; and a heated recovery cage. Verify that the water temperature is consistent with the animal’s body temperature; water that is too hot or cold can cause shock.
- Restraint – Hold the mouse by the base of the tail, supporting the body with the opposite hand. Maintain a relaxed grip to avoid compressing the thorax.
- Wet the coat – Gently immerse the animal’s torso in the water, keeping the head above the surface. Apply a thin film of the surfactant using a soft brush or gauze, moving from dorsal to ventral surfaces.
- Lather – Work the solution through the fur for 10–15 seconds, avoiding the eyes, ears, and genitals. Do not scrub vigorously; the goal is to loosen debris and contaminants.
- Rinse – Slowly pour lukewarm water over the mouse, ensuring complete removal of the cleaning agent. Use a cup or gentle stream; pressure must be low to prevent skin irritation.
- Dry – Transfer the mouse to a towel, pat the fur gently to absorb excess moisture. Place the animal in a pre‑warmed recovery cage with a heat source (e.g., a heating pad set to 30 °C) until it is fully dry and mobile.
After the procedure, inspect the skin for lesions or signs of irritation. Record the duration of the bath, temperature of water, and any adverse reactions. Dispose of used water and cleaning agents according to biosafety protocols. Regular bathing should be limited to cases requiring decontamination or treatment of dermatological conditions; routine cleaning is unnecessary and may disrupt the natural microbiota.