How should I administer ear drops to a rat? - briefly
Restrain the rat, tilt the head to expose the external auditory canal, and apply 1–2 µL of the prescribed otic solution with a calibrated micro‑pipette, ensuring the drop stays within the canal. Observe the animal for 30 seconds to permit absorption, then return it to its cage and monitor for any adverse reactions.
How should I administer ear drops to a rat? - in detail
Accurate delivery of otic medication to a laboratory rat requires preparation, proper restraint, and careful observation.
First, gather all necessary items: sterile otic solution, calibrated micropipette or calibrated dropper, disposable gloves, clean gauze, a soft towel or restraining device, and a record sheet for dosage and time. Verify that the solution is at room temperature; cold liquids may cause vasoconstriction and discomfort.
Second, calculate the appropriate volume. Typical dosing for a rat ranges from 0.02 ml to 0.05 ml per ear, depending on body weight and concentration. Use the animal’s weight (g) to determine the exact amount, applying the formula dose = (0.001 ml × weight kg). Record the calculated volume before proceeding.
Third, restrain the animal. Place the rat on a soft towel, gently but firmly hold the scruff of the neck with one hand while supporting the body with the other. This method minimizes stress and prevents sudden movements.
Fourth, inspect the ear canal. Using a disposable otoscope or a magnifying glass, ensure the canal is free of debris, excessive wax, or lesions. If blockage is present, clear it with sterile gauze before applying medication.
Fifth, administer the drops. Load the predetermined volume into the micropipette, avoiding air bubbles. With the ear upright, gently pull the pinna back to straighten the canal. Place the tip of the pipette just inside the entrance, then release the solution slowly, allowing it to flow into the canal without forcing. Repeat the procedure for the opposite ear if required.
Sixth, observe post‑administration. Keep the rat in a quiet area for at least five minutes to permit absorption and prevent immediate drainage. Monitor for signs of irritation, excessive scratching, or altered behavior. Document any adverse reactions and the exact time of administration.
Finally, dispose of all used materials according to biosafety protocols and clean the work area. Accurate record‑keeping and adherence to the steps above ensure reliable dosing and animal welfare.