How should glue be applied for mice? - briefly
Use a thin, even coat of non‑toxic adhesive on a small, clean surface, place the mouse gently onto it, and allow the glue to set for a few seconds before releasing. Ensure the animal is handled calmly and the amount of glue is minimal to avoid excess residue.
How should glue be applied for mice? - in detail
When attaching adhesive to a laboratory mouse, select a medical‑grade cyanoacrylate or a biocompatible silicone sealant. These compounds cure rapidly, adhere securely, and minimize tissue irritation.
First, prepare the work area. Clean the surface with 70 % isopropyl alcohol, allow it to dry, and ensure ambient temperature is between 20 °C and 25 °C. Wear nitrile gloves to prevent contamination.
Second, restrain the animal using a soft‑foam restrainer or a brief inhalation anesthetic (e.g., isoflurane at 2–3 %). Verify that the mouse is immobile but breathing normally before proceeding.
Third, apply the adhesive:
- Load a fine‑tip micro‑pipette with 0.5–1 µL of the selected glue.
- Position the tip 1–2 mm from the target site (e.g., dorsal scalp, tail base) using a stereomicroscope.
- Deliver a single droplet, avoiding excess that could spread to surrounding fur.
- Allow the material to polymerize for 10–15 seconds; do not disturb the mouse during this interval.
Fourth, post‑application care. Monitor the mouse for 5 minutes to ensure the bond has set and that respiration remains normal. Return the animal to its cage, providing a warm, dry environment. Observe daily for signs of inflammation or detachment; if any adverse reaction occurs, remove the adhesive with a gentle saline rinse and seek veterinary assistance.
Finally, document the procedure: record glue type, volume applied, anatomical location, and any observations. This information supports reproducibility and compliance with animal welfare regulations.