How do you prepare sumamed for a rat? - briefly
Dissolve the calculated amount of azithromycin (Sumamed) in sterile saline to reach the desired concentration, filter‑sterilize, and deliver by oral gavage at 10 mg kg⁻¹. Adjust the dosing volume to approximately 0.5 ml per 100 g of animal weight.
How do you prepare sumamed for a rat? - in detail
Prepare azithromycin for a laboratory rat by following these steps.
Determine the required dose. Typical oral dosing ranges from 10 mg kg⁻¹ to 30 mg kg⁻¹ depending on the experimental protocol. Weigh the animal, calculate the dose in milligrams, and record the value.
Select a suitable vehicle. Dissolve the drug in sterile distilled water or a buffered saline solution (pH 7.2–7.4). Azithromycin is sparingly soluble; add a small amount of 0.1 % (w/v) sodium bicarbonate or 0.5 % (v/v) dimethyl sulfoxide if necessary, ensuring the final concentration does not exceed 10 mg mL⁻¹ to keep the volume for gavage below 0.5 mL per 100 g body weight.
Procedure for solution preparation:
- Weigh the required azithromycin powder using an analytical balance.
- Transfer the powder to a sterile glass vial.
- Add a measured volume of the chosen vehicle, vortex for 30 seconds.
- If precipitation occurs, gently warm the vial to 37 °C and stir until fully dissolved.
- Filter the solution through a 0.22 µm sterile syringe filter into a sterile container.
- Label with concentration, date, and expiration (use within 24 h if stored at 4 °C; longer storage at –20 °C after aliquoting).
Administer the preparation:
- Oral gavage: draw the calculated volume into a calibrated gavage needle, confirm correct placement, and deliver slowly to avoid aspiration.
- Intraperitoneal injection (if protocol permits): ensure the solution is isotonic, inject using a 27‑30 G needle into the lower right quadrant of the abdomen, not exceeding 0.2 mL per 20 g of body weight.
Record the batch number, preparation time, and any deviations. Dispose of waste according to institutional biosafety guidelines.