How to apply drops to a rat's nose? - briefly
Secure the rat gently, tilt its head slightly upward, and dispense 1–2 µL of the solution directly onto the nostril opening using a calibrated pipette, allowing the liquid to be absorbed without forcing. Monitor the animal for any signs of distress and repeat the procedure only after a short recovery period if needed.
How to apply drops to a rat's nose? - in detail
Applying a liquid medication to a laboratory rat’s nasal passages requires precise technique to ensure accurate dosing and minimize stress. The following protocol outlines each stage of the procedure.
First, prepare the work area. Use a clean, well‑lit surface and have all materials within arm’s reach: calibrated dropper or micropipette, sterile solution, disposable gloves, a soft restraining device (e.g., a Plexiglas tube or a small animal restrainer), and a waste container for excess fluid. Verify that the droplet volume matches the prescribed dose; a typical dose ranges from 5 µL to 20 µL per nostril, depending on the study.
Second, acclimate the animal. Allow the rat to rest in the restrainer for 1–2 minutes so that it becomes accustomed to the confinement. Handle the animal gently, supporting the body with one hand while the other hand stabilizes the head.
Third, position the rat for optimal access to the nares. Tilt the head slightly upward (approximately 30°) to open the nasal passages. Ensure the animal’s eyes remain open and the airway is unobstructed.
Fourth, deliver the medication. Load the calibrated dropper with the exact volume. Place the tip just outside the nostril opening, avoiding direct contact with the skin or fur. Release the droplet slowly, allowing it to flow into the nasal cavity by capillary action. If two nostrils require dosing, repeat the process on the opposite side using a fresh tip to prevent cross‑contamination.
Fifth, observe the rat for a brief period (10–15 seconds) to confirm that the fluid remains in the nasal cavity and does not spill onto the fur. If spillage occurs, gently blot the excess with a sterile gauze without pressing on the nose.
Finally, record the administration details: time, exact volume, solution concentration, and any immediate reactions. Return the animal to its home cage, monitor for at least five minutes to detect signs of distress or respiratory difficulty, and document observations in the study log.
Adhering to these steps guarantees consistent delivery of nasal drops, reduces variability in experimental outcomes, and upholds animal welfare standards.