How to apply drops to a rat's ears? - briefly
Hold the rat gently, tilt its head to expose the ear canal, and dispense the prescribed volume of drops with a calibrated pipette, avoiding overflow. Keep the animal upright for several seconds to permit absorption, then observe for any adverse reaction.
How to apply drops to a rat's ears? - in detail
Proper administration of otic solutions to laboratory rodents requires sterile equipment, calibrated dosing devices, and a calm environment to minimize stress. The animal should be restrained gently but securely, using a soft towel or a purpose‑built restrainer that allows access to the auricle without compromising breathing.
Procedure
- Verify concentration and volume of the medication; typical doses range from 5 µl to 10 µl per ear, depending on the formulation.
- Warm the dropper tip briefly (≈ 37 °C) to prevent temperature shock.
- Position the rat in lateral recumbency, with the ear to be treated facing upward.
- Gently pull the pinna back and upward to straighten the ear canal.
- Place a single drop at the canal entrance, allowing the liquid to flow inward by capillary action; avoid touching the tip to the skin.
- Release the pinna and observe the animal for 30–60 seconds to ensure the drop remains within the canal and does not spill.
- Repeat the process for the opposite ear if bilateral treatment is required, using a fresh sterile tip for each side.
Aftercare
- Return the rat to its home cage, providing a dry, warm area for recovery.
- Inspect the treated ears at 5‑minute intervals for signs of irritation, discharge, or blockage.
- Document the exact volume administered, the time of application, and any adverse observations in the laboratory record.
Adherence to these steps guarantees accurate dosing, reduces the risk of injury, and supports reliable experimental outcomes.