How should drops be applied to a rat’s nose?

How should drops be applied to a rat’s nose? - briefly

Place the rat in a supine position, gently restrain the head, and deliver 5–10 µL of the solution onto the nostril with a calibrated micropipette, then keep the animal upright for 30–60 seconds to permit absorption. Avoid excessive pressure to prevent aspiration and ensure the droplet stays within the nasal cavity.

How should drops be applied to a rat’s nose? - in detail

When delivering liquid medication to a rodent’s nasal cavity, follow a strict sequence to ensure accurate dosing and minimize distress.

1. Preparation

   • Use a calibrated micropipette or Hamilton syringe with a 30‑gauge needle.
   • Fill the device with the exact volume required (typically 5–10 µL per nostril).
   • Warm the solution to ambient temperature; cold liquids can cause reflexive sneezing.

2. Animal handling

   • Restrain the rat gently but firmly using a padded tube or a soft cloth wrap, exposing the head without applying pressure to the neck.
   • Verify that the animal is alert, breathing normally, and not exhibiting signs of stress.

3. Positioning

   • Place the animal in a supine or semi‑prone orientation with the head slightly elevated (10–15°).
   • Align the nostrils upward to open the nasal passages.

4. Application

   • Hold the pipette tip just above the external naris, avoiding direct contact with the skin.
   • Release the liquid in a slow, steady stream; the droplet should flow into the nasal cavity by capillary action.
   • Repeat for the opposite nostril if the protocol calls for bilateral dosing.

5. Post‑administration

   • Keep the rat in the inclined position for 30–60 seconds to allow absorption and prevent immediate drainage.
   • Observe for coughing, sneezing, or abnormal breathing; intervene if any adverse reaction occurs.
   • Return the animal to its cage, monitor for at least 5 minutes before resuming normal activities.

6. Documentation

   • Record the exact volume, concentration, time of administration, and any observed reactions.
   • Note the identity of the operator and the equipment used for traceability.

Adhering to these steps guarantees precise nasal delivery, reduces variability between subjects, and upholds animal welfare standards.