How should Baytril be injected into a rat? - briefly
Administer Baytril subcutaneously at 2–5 mg kg⁻¹, using a sterile 1‑ml syringe fitted with a 27–30 G needle, and inject into the dorsal flank away from the tail. Dilute the veterinary preparation with sterile saline as needed to achieve the calculated dose.
How should Baytril be injected into a rat? - in detail
Baytril (enrofloxacin) administration to laboratory rats requires precise preparation and technique to ensure accurate dosing and minimize tissue trauma.
The recommended dose for systemic infection control ranges from 5 mg kg⁻¹ to 10 mg kg⁻¹, delivered once daily. Calculate the required amount based on the animal’s exact weight; for a 250 g rat, a 5 mg kg⁻¹ regimen equals 1.25 mg of active ingredient.
Preparation steps
- Reconstitute the lyophilized powder with sterile 0.9 % sodium chloride or sterile water for injection, following the manufacturer’s instructions.
- Verify the final concentration; a common preparation yields 10 mg mL⁻¹, allowing a 0.125 mL injection for the example above.
- Filter the solution through a 0.22 µm sterile filter if the product is not pre‑filtered.
Injection technique
- Restrain the rat using a gentle, approved method (e.g., an acrylic restrainer) to avoid stress and movement.
- Select a 27‑ to 30‑gauge needle with a 0.5‑ to 1‑inch length, appropriate for the calculated volume.
- Identify the intraperitoneal site: a 1‑cm lateral offset from the midline, just caudal to the umbilicus, avoids major organs.
- Insert the needle bevel‑up at a shallow angle (≈30°) into the peritoneal cavity, confirming entry by a slight “give” sensation.
- Inject the prepared solution slowly (≈0.1 mL s⁻¹) to prevent sudden pressure changes.
- Withdraw the needle smoothly, apply gentle pressure with sterile gauze to the entry point, and release the animal into a warm recovery cage.
Post‑injection monitoring
- Observe the rat for at least 30 minutes for signs of distress, abnormal respiration, or injection‑site reactions.
- Record the administered volume, concentration, and any adverse observations in the animal’s study log.
Adherence to aseptic technique, accurate dosing calculations, and proper intraperitoneal placement ensures the effective delivery of « Baytril » in rodent models.