How can a mouse repellent be tested? - briefly
Set up identical test chambers, introduce a known number of rodents, apply the deterrent in one chamber, and compare capture rates or movement patterns over a defined period. Statistical analysis of the differences determines efficacy.
How can a mouse repellent be tested? - in detail
Testing a mouse deterrent requires a repeatable protocol that isolates the product’s effect from external variables. The procedure begins with selecting a suitable test arena, such as a sealed Plexiglas enclosure measuring 60 × 30 × 30 cm, equipped with bedding, food, and a shelter. Two identical arenas are prepared: one receives the repellent, the other serves as an untreated control.
A cohort of laboratory‑bred mice, balanced for sex and age, is introduced simultaneously into both arenas. Each group contains at least ten individuals to provide statistical power. The repellent is applied according to the manufacturer’s instructions—spray, pellet, or ultrasonic device—ensuring uniform coverage of the test surface.
Key performance indicators include:
- Entry frequency: count of mice that cross a predefined line toward the treated zone within a 24‑hour period.
- Time spent: cumulative minutes each mouse remains in the treated area, recorded by infrared motion sensors.
- Food consumption: weight of food removed from the treated versus control arena, measured before and after the test.
- Stress markers: cortisol levels obtained from fecal samples collected at the start and end of the trial.
Data are logged continuously using a data‑acquisition system. After 48 hours, mice are removed, arenas are cleaned, and the experiment is repeated three times to verify reproducibility. Statistical analysis employs a two‑sample t‑test or Mann‑Whitney U test, depending on data distribution, with a significance threshold of p < 0.05.
Environmental conditions—temperature (22 ± 2 °C), humidity (50 ± 10 %), and lighting cycle (12 h light/12 h dark)—are maintained constant across all trials. Any deviation is documented and factored into the analysis.
Safety considerations include wearing protective gloves when handling chemical repellents and ensuring ultrasonic devices do not exceed recommended intensity levels to avoid auditory damage to personnel.
The final report presents mean values, confidence intervals, and effect sizes for each metric, allowing a clear assessment of the product’s efficacy relative to the untreated baseline.