How to restrain a mouse? - briefly
Place the mouse in a clear, ventilated container with a snug‑fitting lid and hold the lid gently to prevent movement during handling. Use fine forceps or a soft brush to guide the animal into the container without causing injury.
How to restrain a mouse? - in detail
Effective immobilization of a laboratory mouse requires preparation, appropriate equipment, and adherence to humane standards.
Before handling, verify that personal protective equipment (gloves, lab coat) is worn and that the work area is clean. Ensure the animal is healthy and acclimated to reduce stress.
Select a suitable restraining device based on the intended procedure. Common options include:
- Transparent plastic tube (15–20 cm long, 2–3 cm diameter) with open ends; allows visual observation while limiting movement.
- Soft fabric restraining bag; folds over the animal, securing the head with a gentle pinch.
- Specialized mouse restrainer with adjustable clamps; immobilizes limbs without excessive pressure.
- Anesthetic chamber for chemical immobilization; used when prolonged restraint is necessary.
The manual handling technique follows a consistent sequence:
- Approach the mouse calmly, avoid sudden movements.
- Grasp the base of the tail with thumb and forefinger, supporting the body with the opposite hand.
- Transfer the animal into the chosen device, ensuring the head remains accessible if required.
- Secure the device according to manufacturer instructions, confirming that breathing is unobstructed.
During restraint, monitor respiratory rate and limb coloration. If signs of distress appear, release the animal immediately and reassess the method.
After completing the procedure, place the mouse in a clean recovery cage with access to food and water. Record the duration of immobilization and any adverse observations for future reference.
Adhering to these steps minimizes injury risk, ensures reliable experimental conditions, and upholds ethical standards for rodent handling.