How to determine the sex of a domestic rat?

How to determine the sex of a domestic rat? - briefly

Inspect the underside: males show a scrotum and a noticeably longer, pointed anogenital distance, whereas females have a smooth, rounded opening without a scrotum. The anogenital separation in males is roughly twice that of females.

How to determine the sex of a domestic rat? - in detail

Identifying the gender of a pet rat requires careful visual inspection of the ventral region just behind the anus. The following steps outline the procedure for both juvenile and adult specimens.

  1. Prepare the animal

    • Gently restrain the rat by holding the base of its tail with one hand and supporting its body with the other.
    • Use a calm environment to minimize stress; a soft towel can provide a secure surface.
  2. Locate the anogenital area

    • Turn the rat onto its back or side. The opening, called the genital papilla, is positioned midway between the anus and the base of the tail.
  3. Examine external characteristics

    • Males

      • A visible bulge or scrotal sac lies beneath the vent, often appearing as a small, rounded swelling.
      • The distance between the anus and the genital papilla (the anogenital distance) is noticeably longer than in females.
      • In mature individuals, the testes may be palpable as firm, rounded masses within the scrotum.
    • Females

      • The genital papilla is a flat, smooth area without a protruding bulge.
      • The anogenital distance is short, typically less than 5 mm in young rats and remaining relatively consistent in adults.
      • No scrotal sac or testes are present; the area feels smooth to the touch.
  4. Confirm with age‑appropriate cues

    • In pups younger than 10 days, the anogenital distance remains the most reliable indicator because testes have not descended.
    • In rats older than 3 weeks, the presence of a scrotal sac provides unequivocal confirmation.
  5. Record findings

    • Note the sex, age, and any observable anomalies (e.g., cryptorchidism, where testes fail to descend).
    • Maintain consistent documentation for breeding or veterinary purposes.

Accurate gender determination is essential for proper breeding management, health monitoring, and behavioral assessment of domestic rats.