How should Cipromed be administered to a rat? - briefly
Administer Cipromed to rats by oral gavage, using a sterile aqueous or mild‑oil vehicle at 10–20 mg kg⁻¹ body weight, once daily. Prepare a uniform suspension and deliver it with a calibrated feeding needle to prevent aspiration and ensure accurate dosing.
How should Cipromed be administered to a rat? - in detail
Cipromed must be delivered to a rat using a well‑defined protocol that ensures accurate dosing, sterile technique, and minimal stress to the animal.
First, prepare a solution at the concentration required for the intended dose. Dissolve Cipromed in a suitable vehicle—commonly sterile saline or phosphate‑buffered saline—adjusting pH to 7.2–7.4 if necessary. Filter the mixture through a 0.22 µm membrane to remove particulates. Store the solution at 2–8 °C and use within 24 h to maintain stability.
Select the route of administration based on experimental objectives:
- Intraperitoneal (IP) injection – most common for systemic exposure. Use a 26‑ to 27‑gauge needle, inject into the lower right quadrant of the abdomen, avoiding the midline and major organs. Advance the needle at a shallow angle (≈30°) until a slight “pop” is felt, indicating entry into the peritoneal cavity. Deliver the calculated volume (typically ≤10 mL kg⁻¹) slowly to prevent reflux.
- Subcutaneous (SC) injection – appropriate for prolonged absorption. Pinch a loose area of dorsal skin, insert the needle bevel up at a 45° angle, and deposit the dose in the subdermal space. Volume should not exceed 5 mL kg⁻¹.
- Intravenous (IV) injection – reserved for precise pharmacokinetic studies. Cannulate the lateral tail vein with a 30‑gauge catheter, confirm patency by observing blood return, and inject the dose over 30–60 seconds. Use a volume ≤5 mL kg⁻¹ to avoid circulatory overload.
Calculate the dose in mg kg⁻¹, then convert to the required volume using the prepared concentration. Record the exact amount administered for each animal. Prior to injection, warm the rat for 2–3 minutes to dilate peripheral vessels if IV access is needed.
After delivery, monitor the rat for at least 30 minutes. Observe for signs of distress, abnormal respiration, or injection site reactions. Document body weight, temperature, and any adverse events. If repeated dosing is planned, maintain consistent timing (e.g., every 24 h) and use the same route and injection technique throughout the study.
Maintain a detailed log that includes animal identifier, date and time of administration, dosage, route, needle gauge, volume, and post‑administration observations. This record supports reproducibility and regulatory compliance.