How can I administer an injection to a rat in the scruff? - briefly
Hold the rat securely by pinching the loose skin on the back of its neck and tilt the head slightly upward. Insert the needle at a 30‑45° angle into the chosen site (subcutaneous or intraperitoneal) and deliver the dose.
How can I administer an injection to a rat in the scruff? - in detail
To deliver an injection safely, first gather sterile equipment: appropriate‑size needle and syringe, the chosen drug, antiseptic wipes, and a clean work surface. Verify dosage, label the syringe, and check for air bubbles before use.
Restrain the animal by firmly grasping the loose skin on the dorsal neck region between thumb and forefinger. Apply gentle upward pressure to lift the head, creating a natural extension of the spine. This grip immobilizes the rat without causing distress. Maintain the hold for the entire procedure; release only after the needle is withdrawn.
Select the injection site based on the required route:
- Intraperitoneal (IP): Insert the needle at a 30‑45° angle into the lower right quadrant of the abdomen, avoiding the bladder and large intestines. Advance until a slight “pop” is felt, indicating entry into the peritoneal cavity.
- Subcutaneous (SC): Pinch a fold of loose skin on the dorsal flank, then insert the needle at a shallow 10‑15° angle, directing the tip into the subdermal space.
- Intramuscular (IM): Locate the hind‑limb thigh muscle (quadriceps). Insert the needle perpendicular to the skin, penetrating the muscle tissue fully.
Administer the medication slowly, observing for resistance or leakage. After delivery, withdraw the needle smoothly and apply gentle pressure with a sterile swab to the puncture site to minimize bleeding. Release the animal from the scruff and place it in a recovery cage with easy access to food and water.
Clean and disinfect all reusable tools, dispose of sharps in a puncture‑proof container, and document the procedure, including drug name, dose, route, and any observations.