How can a rat be simply stitched? - briefly
A simple stitch is performed with a sterile needle and thread, placing interrupted sutures 2–3 mm apart to close the wound. Apply a mild antiseptic and observe the site for signs of infection.
How can a rat be simply stitched? - in detail
Suturing a laboratory rat requires anesthesia, aseptic technique, appropriate instruments, and careful post‑operative management.
Anesthesia is induced with inhaled isoflurane or an injectable combination of ketamine (80 mg/kg) and xylazine (10 mg/kg). Confirm depth of anesthesia by lack of pedal reflex before proceeding.
The surgical site is shaved, cleaned with 70 % ethanol, and draped with sterile gauze. Instruments include a microsurgical needle holder, fine forceps, and monofilament suture of 6‑0 or 7‑0 nylon.
The incision is made with a scalpel blade, typically 5–8 mm in length. For wound closure:
- Place two simple interrupted sutures at each end of the incision, spacing 2–3 mm apart.
- Insert the needle perpendicular to the skin, passing through the dermis on each side.
- Tie a square knot with three throws, maintaining tension to approximate edges without strangulation.
- If the wound is longer, add additional interrupted sutures at regular intervals, preserving consistent spacing.
- For deeper layers, a single buried mattress suture may be employed using the same suture material.
After closure, apply a sterile saline rinse and a thin layer of topical antibiotic ointment.
Analgesia is provided with buprenorphine (0.05 mg/kg subcutaneously) every 12 hours for 48 hours. Monitor the animal for signs of pain, infection, or dehiscence, and record body temperature and weight daily for the first three days.
If any suture loosens or the wound opens, re‑suture promptly using the same technique. Proper documentation of the procedure, including suture material and knot configuration, ensures reproducibility and compliance with institutional animal care guidelines.